AP® Biology · Unit 3 · Topic 3.5
Fermentation
Fermentation is a way to regenerate NAD+ from NADH so glycolysis can keep running when no oxygen is available. It produces no ATP itself; all the ATP comes from glycolysis.
Glycolysis splits glucose into two pyruvate molecules and nets 2 ATP, but it only keeps running if there's enough NAD+ around to accept electrons. Normally that NAD+ gets recycled when NADH hands its electrons off to the electron transport chain, which needs oxygen as the final acceptor. Cut off the oxygen, and NADH piles up while NAD+ runs out. Glycolysis stalls. Fermentation is the patch: it takes the electrons back off NADH and dumps them onto pyruvate (or something made from pyruvate), turning NADH back into NAD+. That's the entire job. No ATP synthase, no proton gradient, no substrate-level phosphorylation happens in fermentation itself.
Animal cells, including your muscle cells during a hard sprint, run lactic acid fermentation: pyruvate accepts the electrons directly and becomes lactate, in one step. Yeast and some bacteria run alcoholic fermentation instead: pyruvate first loses a carbon as CO2, becoming acetaldehyde, and then acetaldehyde accepts the electrons from NADH and becomes ethanol. Both pathways regenerate the same NAD+ that glycolysis needs, just with different final products.
This sits right next to aerobic respiration in the unit: same starting point, glycolysis, but a different ending depending on whether oxygen is present to keep the electron transport chain running. Fermentation is slower to generate ATP per glucose but doesn't depend on oxygen at all, which is why a sprinting muscle, starved for oxygen, leans on it, while a resting, well-oxygenated muscle doesn't need to.
Key terms
- NAD+/NADH
- An electron carrier molecule; NAD+ accepts electrons to become NADH, and must be regenerated for glycolysis to continue.
- Substrate-level phosphorylation
- Direct transfer of a phosphate group to ADP from another molecule, making ATP without a membrane or electron transport chain.
- Lactic acid fermentation
- Pathway where pyruvate directly accepts electrons from NADH and becomes lactate, regenerating NAD+.
- Alcoholic fermentation
- Pathway where pyruvate loses CO2 to form acetaldehyde, which then accepts electrons from NADH to become ethanol.
Watch it explained
- How it worksHow does fermentation recycle NAD plus so glycolysis can keep running without oxygen?
- How it worksWalk me through pyruvate becoming lactic acid versus becoming ethanol and carbon dioxide.
- Going furtherWhat happens to a yeast culture's carbon dioxide output when you take its oxygen away?
- Common mistakeWhy doesn't fermentation itself produce any ATP?
Check yourself
End of topic.