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AP® Biology · Unit 2 · Topic 2.5

Passive and Active Transport

Passive transport moves solutes down their concentration gradient using only random thermal motion, while active transport uses energy to push solutes against their gradient. Diffusion rate scales directly with gradient size.

Every molecule in a solution jiggles constantly from its own thermal energy, bumping around in random directions with no plan. A drop of dye in still water spreads because there are simply more ways for dye molecules to end up spread out than clumped together. Early on, nearly all the random hits send dye molecules from the crowded spot toward the empty water, because that's where most of the open space is. That lopsided random motion is diffusion, and it needs no stirring and no energy input, just time and heat.

Once dye concentration is equal everywhere, the molecules do not stop moving. They keep jiggling with the same thermal energy as always. What stops is the net flow: molecules now cross any imaginary boundary in both directions at equal rates, so the system looks unchanging even though it is busy underneath. This is dynamic equilibrium, and it's the detail students most often get wrong, picturing frozen molecules instead of balanced traffic.

Moving a solute uphill, from low to high concentration, fights this natural spread, so a cell cannot rely on random motion alone. It needs a transport protein built to bind the solute and change shape, and it needs a source of energy, usually ATP, to drive that shape change. This is active transport, and it sits right next to diffusion and osmosis in the bigger story of the membrane: passive methods handle gradients that already favor movement, active methods handle the gradients a cell must fight to maintain, like the steep sodium and potassium gradients neurons depend on.

Key terms

Diffusion
The net movement of molecules from high to low concentration caused by random thermal motion.
Dynamic equilibrium
The state where molecules keep moving but cross a boundary at equal rates in both directions, giving zero net change.
Passive transport
Movement of a solute down its concentration gradient that requires no cellular energy input.
Active transport
Movement of a solute against its concentration gradient, requiring a transport protein and an energy source like ATP.
Concentration gradient
The difference in solute concentration between two regions, which determines the direction and drive for diffusion.

Watch it explained

  1. How it worksHow does purely random jiggling add up to a net flow in one direction?
  2. Common mistakeWhy doesn't molecular motion stop once concentrations even out on both sides?
  3. How it worksWalk me through what has to change when a cell moves a solute uphill instead.
  4. Going furtherWhat happens to the net rate of diffusion when the gradient across a membrane doubles?

Check yourself

Your turn1 of 2
Why doesn't molecular motion stop once concentrations even out on both sides of a membrane?

End of topic.